Research

Enhancing Reproducibility in Cancer Drug Screening: How Do We Move Forward?

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Abstract

Large-scale pharmacogenomic high-throughput screening (HTS) studies hold great potential for generating robust genomic predictors of drug response. Two recent large-scale HTS studies have reported results of such screens, revealing several known and novel drug sensitivities and biomarkers. Subsequent evaluation, however, found only moderate interlaboratory concordance in the drug response phenotypes, possibly due to differences in the experimental protocols used in the two studies. This highlights the need for community-wide implementation of standardized assays for measuring drug response phenotypes so that the full potential of HTS is realized. We suggest that the path forward is to establish best practices and standardization of the critical steps in these assays through a collective effort to ensure that the data produced from large-scale screens would not only be of high intrastudy consistency, so that they could be replicated and compared successfully across multiple laboratories.

drug response phenotype drug sensitivity genome Primates Mammalia Vertebrata Chordata Animalia (Animals Chordates Humans Mammals Primates Vertebrates) - Hominidae [86215] human common biomarker 10062 Biochemistry studies - Nucleic acids purines and pyrimidines 12512 Pathology - Therapy 22002 Pharmacology - General 22005 Pharmacology - Clinical pharmacology 24004 Neoplasms - Pathology clinical aspects and systemic effects cancer drug screening laboratory techniques large-scale pharmacogenomic high-throughput screening laboratory techniques Methods and Techniques Pharmacology Tumor Biology ONCOLOGY MICROARRAY DATA CYTOTOXICITY INHIBITION DISCOVERY SENSITIVITY STANDARDS MELANOMA STRATEGY IMPACT TARGET Reviews