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Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production

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Abstract

We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R-2 = 0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R-2 = 0.4269). All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform. (C) 2014 Elsevier Ltd. All rights reserved.

Denmark Europe Palearctic region enzymatic saccharification lignocellulosic biomass recalcitrant feedstock Monocotyledones Angiospermae Spermatophyta Plantae (Angiosperms Monocots Plants Spermatophytes Vascular Plants) - Gramineae [25305] winter wheat common cultivar-Flair cultivar-Penso cultivar-Abika cultivar-Oakley cultivar-Inspiration cultivar-Audi cultivar-Skalmeje cultivar-Jenga cultivar-Potenzial cultivar-Glasgow cultivar-Dinosor cultivar-Smuggler cultivar-Ambition cultivar-Hattrick cultivar-Florett cultivar-Tommi cultivar-Samyl cultivar-Tuscan cultivar-Robigus cultivar-Opus cellulose 9004-34-6 ethanol 64-17-5 fermentable sugars glucose 58367-01-4 xylose 25990-60-7 00512 General biology - Conservation and resource management 04500 Mathematical biology and statistical methods 10060 Biochemistry studies - General 10068 Biochemistry studies - Carbohydrates 39008 Food microbiology - General and miscellaneous 51522 Plant physiology - Chemical constituents Computational Biology advanced high throughput pretreatment system laboratory techniques enzymatic hydrolysis system laboratory techniques high throughput screening method laboratory techniques statistical analysis mathematical and computer techniques Biochemistry and Molecular Biophysics Bioprocess Engineering Conservation Mathematical Biology AGRICULTURAL BIOTECHNOLOGY ENERGY WHEAT-STRAW PRETREATMENT TECHNOLOGIES ENZYMATIC-HYDROLYSIS CELLULOSIC ETHANOL CORN STOVER IMPACT High throughput screening Lignocellulosic ethanol Wheat straw Genetic selection Microscale pretreatment Enzymatic hydrolysis Lignocellulosic ethanols Pre-Treatment Wheat straws Cellulosic ethanol