In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis
Abstract
SILAC technology in combination with high-resolution mass spectrometry (MS) can be successfully used to measure phosphoproteomes in vivo. Here, Zanivan, Mann, and colleagues have applied SILAC-based MS to investigate phosphoproteomic changes during skin carcinogenesis, using the DMBA/TPA two-stage mouse model. Using this approach, the authors have revealed the phosphoproteomic dynamics that accompany skin cancer progression and predict specific kinase activities associated with tumor malignancy.
Info
Journal Article, 2013
UN SDG Classification
DK Main Research Area
Science/Technology
