Influence of protein source on amino acid uptake patterns and protein utilization in rainbow trout Oncorhynchus mykiss
Abstract
Matrixes of different protein sources (fish and plant products) combined with the use of crystalline amino acids allow for formulation of diets that meet fish requirements with little or no effect on protein digestibility and/or feed intake. Despite this, a total or partial replacement of fish meal induces reduced growth performances that remain partly unexplained. The aim of the current study was to investigate the effect of exchanging the protein source on protein utilization. Marine (fish meal) and vegetable (pea protein) sources were used with or without supplementation of crystalline amino acids to the fishmeal diet level (see Table 1). Amino acid uptake patterns were assessed by the appearance of amino acids in the blood stream following the ingestion of a meal, while dietary protein utilization was evaluated by examining the metabolic response to digestion and ammonium and urea excretion rates during digestion. Four treatments, 3 diets and 1 control (no feeding), were applied to rainbow trout with an average body mass of 500 grams. Fish were either force fed one of the 3 diets at a ration corresponding to 0,75% of the body mass, or no force feeding. Four fish at a time (one per treatment) were placed in individual chambers for 48h. Blood and water samples were collected at time 0 and then at 2, 4, 6, 8, 12, 20, 32 and 48 hours post feeding. The protocol was repeated until 8 replicates per treatment were obtained The results were obtained through 2 separate experiments. In the first part, oxygen consumption was recorded continuously, while water was sampled as detailed above and analyzed for ammonium and urea content. The second part of the experiment was designed to collect blood samples. After the feeding treatment fish were held in separate containers for the above described time sampling. Fish were killed by a blow in the head and blood was collected from the caudal vein with heparinized syringes. Plasma and red blood cells content were stored separately at -80 for amino acid content analysis. The ammonium excretion profiles (Figure 1) will be correlated with the amino acid profile in the blood and oxygen consumption during digestion to investigate the effect on protein utilization for each treatment
Info
Conference Abstract, 2012
UN SDG Classification
DK Main Research Area
Science/Technology
