Single-cell analysis by inductively coupled plasma- time-of-flight mass spectrometry to quantify algal cell interaction with nanoparticles by their elemental fingerprint
Abstract
Single-cell analysis has recently become a growing research field, and has found many applications in a variety of disciplines such as biochemistry, toxicology, metallomics, and even medical diagnosis and pharmaceutics for drug and cancer research. Although several analytical methods exist for the analysis of individual cells and their cellular content, this article focuses on highlighting the latest applications and trends in which inductively coupled plasma-time-of-flight mass spectrometry (ICPTOF- MS) is applied to single-cell analysis. The novelty of this method relies on the determination of the elemental fingerprint through the detection ofelements intrinsically present in cells. By always measuring a full mass spectrum, there is no need for compromising which analytes are measured, and nothing is missed. This is particularly beneficial for nanotoxicology studies, where a direct and complete picture of the nanoparticle-cell association is provided.
Info
Journal Article, 2020
UN SDG Classification
DK Main Research Area
Science/Technology
