Stable isotope-resolved analysis with quantitative dissolution dynamic nuclear polarization
Abstract
Metabolite profiles and their isotopomer distributions can be studied non-invasively in complex mixtures with NMR. The advent of dissolution Dynamic Nuclear Polarization (dDNP) and isotope enrichment add sensitivity and resolution to such met-abolic studies. Metabolic pathways and networks can be mapped and quantified if protocols that control and exploit the ex situ sig-nal enhancement are created. We present a sample preparation method, including cell incubation, extraction and signal enhance-ment, to facilitate reproducible and quantitative dDNP (qdDNP) NMR-based isotope tracer analysis. We further illustrate how qdDNP was applied to gain systematic and novel metabolic phenotypic insights into aggressive cancer cells.
Info
Journal Article, 2018
UN SDG Classification
DK Main Research Area
Science/Technology
