β-Hydroxybutyrate dehydrogenase decorated MXene nanosheets for the amperometric determination of β-hydroxybutyrate
Abstract
MXene nanosheets of type Ti3C2Tx were modified with β-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of β-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme β-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of − 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 μA mM−1 cm−2, and a low detection limit (45 μM). The biosensor was successfully applied to the determination of β-hydroxybutyrate in (spiked) real serum samples. [Figure not available: see fulltext.].
Info
Journal Article, 2020
UN SDG Classification
DK Main Research Area
Science/Technology
